Microfluidic chip is usually also known as biochip. Microfluidic technology can integrate the entire experimental process and functions in conventional biochemical analysis, such as sampling, dilution, reagent addition, reaction, separation, and detection, into a small solid-phase material such as silicon, glass, plastic, or metal. A microchip including multiple micro-nano-pipes and multiple micro-nanoliter-volume reaction chambers runs through the entire system with controllable fluids to achieve various functions in conventional chemical or biological laboratories. Serving applications such as point-of-care diagnostics (POCT), gene sequencing, environmental protection, food safety, and scientific research.
Existing rapid diagnostic methods such as colloidal gold and immunochromatography have low sensitivity and specificity and poor reproducibility, whereas microfluidic technology can realize precise quantitative processing and manipulation of micro-liquids in micrometer-sized channels, which can achieve the accuracy and sensitivity of large-scale detectors.
Multiple flow channels can be designed on the microfluidic chip, and the samples to be detected can be assigned to multiple reaction units at the same time through the microfluidic channel network, while the reaction units are isolated from each other and do not interfere with each other. Not only can the same sample be tested for multiple items in parallel, but also multiple samples can be processed at the same time, realizing high-throughput detection.
Through the design combination of microfluidic channels, micro-pumps, micro-valves and cavities, microfluidic technology is able to realize multiple steps required for sample testing on a chip. Since the testing instrument does not need to manipulate the fluid, it not only realizes the miniaturization and automation of the whole machine, but also saves operation time and improves the speed of testing and diagnosis.
The characteristic scale of microfluidic chips is on the micron scale, and the reaction unit chambers are tiny, with volumes ranging from microliters to nanoliters or even picoliters. Although the concentration of the reagent formula may be increased by a certain percentage, the amount of reagent used is far lower than that of conventional detection methods, which greatly reduces the consumption of reagents.
Microfluidic chips are characterized on a micron scale, with tiny reaction unit chambers and volumes ranging from microliters to nanoliters and even to picoliters. Although the concentration of the reagent formulation may be proportionally higher, the amount of reagent used is much lower than that of conventional detection methods, greatly reducing reagent consumption.
The sealed flow channel not only avoids the contamination of samples by manual operation, but also avoids the pollution of samples to the environment. Because the microfluidic chip integrates all the operations that need to be done manually in the traditional laboratory into the chip for automatic completion, and is manufactured and sealed in a clean environment